Viability Assay Kit

Product Name

Viability Assay kit

Cat no.

B1007

Standard

5 mL

Product Explanation

Test our new and more sensitive colorimetric cell viability assay!

- Highest sensitivity
- Less cellular toxicity
- Easier and quicker : One ready-to-use bottle
- Re-use of cells after colorimetric measurement
- Viability analysis on classical or organoid cell culture

Highest Sensitivity

Higher know-how with MediFab’s special know-how
(Uses same ingredients as D Company’s WS-8)

Free sample for 100 tests!
No Toxic to Cell

Safe for both cell and experimenter → No need to use DMSO!

Cells staying healthy in 24-hour cellular reaction

Supports multiple time point assay

Supports additional tests such as gene and protein analysis after test
Flexibility

Comparing OD value for cell count using HL-60 cell (floating cell) → Higher accuracy

Applicable on suspension cell!

Applicable for checking cytotoxicity of various drugs and material extracts!

For checking cytotoxicity!

1 year in refrigerator / 2 years in freezer

Long stability

Simple & Fast

Just take out from refrigerator!

Just add-and-read의 one bottle kit / One bottle kit for just add-and-read / Check the result within a hour!

Procedure	Preparation	Add	Dissolve	Measure	Handling Time
Cellix (Medifab)	-	Add reagent 5min	-	Measure 5min	10min
MTS WST-1	Thaw Reagent 5min	Add reagent 5min	-	Measure 5min	15min
MTT	Prepare reagent 5min	Add reagent 5min	Dissolve MTT 20min	Measure 5min	45min

Q&A

Q

Where can I apply this?

It is applicable on almost all cells (except plant cell)
Q

Recommended cell number & incubation time

Proliferation assay :

Adherent cell - 10,000 cells/well/1~2 hrs, Suspension cell - 2 x 104 ~ 105 cells/well/3~4 hrs

Cytotoxicity assay :

Adherent cell - 5,000 cells/well/1~2 hrs, Suspension cell - 104 ~ 105 cells/well/3~4 hrs
Q

Is there any influence of phenol red?

There is no intervention from phenol red in culture media
Q

How long can I store this?

You can keep it for 1 year in a refrigerator and 2 years in freezer
Q

Is the cell toxic?

The agent involves almost no toxicity and it does not damage cell even after long incubation time After the test, it can be re-used on other tests such as neutral red, crystal violet assay, and DNA fluorometric assay
Q

What wavelength is used for measurement?

Optimum wavelength is 450nm
Q

Is this applicable on 48-well or 24-well?

Yes you can. Add media in 1:10 x

Product components : Cellrix® Viability Assay Kit

Component	Assay	Catalog No.
5ml x 1	500test	B1007-500
5ml x 3	1500test	B1007-1500
5ml x 5	2500test	B1007-2500